Researchers in the US have adapted a DNA amplification technique to develop a simpler way to rapidly screen chemical reactions. The process should improve reaction screening methods known as 'chemical speed-dating' - where hundreds of different substrates are mixed together but only a few interact strongly.
These reaction screens are a great way to quickly probe chemical space to discover new reactions, and this new technique builds on the polymerase chain reaction (PCR) to eliminate some awkward processing steps, boost efficiency and accuracy.
The speed-dating process works by attaching hundreds of different substrates to short, single strands of DNA. When warmed to 60°C, the strands are too short to pair up naturally - but where two substrates have reacted, their DNA tails pair up, forming a short piece of DNA that resembles a hairpin.
Traditionally, one of the substrates typically needs to be mounted on a bead to isolate and identify the compounds that have reacted, which can be a time-consuming practice. Now, David Liu's team at Harvard University, US, have simplified the method by modifying the polymerase chain reaction (used to copy single strands of DNA millions of times) to selectively replicate only the DNA strands where a reaction has occurred.
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